DQC-3/DQC-50 - Instruction Sheet

The Isohelix DQC kit is a PCR kit designed to check the quality and presence of the DNA before you start your full research or testing program. The DQC kit can also be used to compare results against different types of DNA Isolation and the presence of PCR inhibitors.

Expected band sizes: 100, 200, 300, 400, 500 and 600 bp.

If all 6 fragments are observed the DNA is not denatured.

The 500 bp fragment is derived from an internal control and should always be present (even in negative controls). If not, the PCR has failed and needs repeating.

• Different  band intensities can represent different amounts of DNA.

• If less than 6 fragments are seen the DNA is probably partially degraded.

• If only the 500 bp control fragment is seen the DNA is de-natured or has not been added. 

1. 100bp ladder
2. Good Quality DNA – 6 bands present
3. Good Quality DNA – 6 bands present but lower yield so bands less intense
4. Good Quality DNA – 6 bands present
5. Poor Quality DNA – uneven amplification indicates partially degraded DNA
6. Good Quality DNA – 6 bands present
7. Poor Quality DNA – some bands missing
8. Good Quality DNA – 6 bands present
9. Negative Control – 500bp internal control shows PCR worked

For further information please email info@isohelix.com.

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